{"id":8268,"date":"2023-04-28T09:42:38","date_gmt":"2023-04-28T02:42:38","guid":{"rendered":"https:\/\/dynatech-int.com\/?p=8268"},"modified":"2023-06-27T09:47:22","modified_gmt":"2023-06-27T02:47:22","slug":"teknologi-peakforce-tapping-mode-untuk-imaging-dna","status":"publish","type":"post","link":"https:\/\/dynatech-int.com\/id\/teknologi-peakforce-tapping-mode-untuk-imaging-dna\/","title":{"rendered":"Teknologi PeakForce Tapping Mode untuk Imaging DNA"},"content":{"rendered":"\n

Molekul biologis pertama yang dividualisasikan oleh Atomic Force Microscopy (AFM)<\/a> ialah DNA. Hal ini dilakukan untuk mempelajari struktur, topologi, dinamika DNA, dan bagaimana interaksinya dengan protein. Teknologi PeakForce Tapping Mode ekslusif dari Bruker <\/a>dapat melakukan imaging double helix DNA dengan resolusi tinggi yang dapat mengukur kekuatan imaging tanpa memerlukan probe khusus ataupun design AFM yang terbatas.<\/p>\n\n\n\n

Pengenalan AFM ke dalam penelitian Biologi<\/h2>\n\n\n\n

Pada awal tahun 90-an pengenalan TappingMode meningkat secara signifikan dalam penggunaan AFM untuk penelitian biologi. Dalam TappingMode, probe berosilasi pada frekuensi resonansi fundamental dengan posisi vertikal dari tip (atau sampel) kemudian disesuaikan untuk mempertahankan amplitude osilasi yang konstan saat probe melakukan scan melewati surface.<\/p>\n\n\n\n

Sayangnya, Tapping Mode menghasilkan resolusi gambar lebih rendah dibandingkan Contact Mode AFM. Untuk itu, faktor utama dalam meraih hasil gambar AFM berkualitas tinggi membutuhkan kontrol gaya interaksi antara probe dan sampel selama proses imaging.<\/p>\n\n\n\n

Tahun 2010, Bruker memperkenalkan PeakForce Tapping Mode dalam AFM. Metode yang digunakan secara luas dalam penelitian biomolekuler sebab kemampuannya menghasilkan gambar beresolusi tinggi secara konsisten. <\/p>\n\n\n\n

Pada PeakForce Tapping, jarak antara probe dan sampel dimodulasi secara sinusoidal dengan mempertahankan gaya maksimum antara keduanya.<\/p>\n\n\n

\n
\"PeakForce
Gambar 1. In PeakForce Tapping Mode the AFM probe is modulated at low frequency (1-2 kHz). (A) As the probe is brought into contact with the surface, the feedback signal is the maximum or \u201cpeak\u201d force applied to the surface. (B) If the motion of the probe is considered in terms of Z position, one is essentially performing a force curve at every position of the sample surface<\/figcaption><\/figure><\/div>\n\n\n

Feedback loop waktu secara nyata digunakan untuk mengontrol posisi probe dan penggunaan sinusoidal ramping sehingga kecepatan scanning lebih tinggi. Bahkan, teknologi PeakForce Tapping menggunakaan mode imaging ScanAsyst yang mengoptimalisasi setpoint imaging secara otomatis.<\/p>\n\n\n\n

Metode PeakForce Tapping Mode pada DNA<\/h2>\n\n\n\n

Metode PeakForce Tapping Mode berhasil melakukan imaging DNA dengan resolusi tinggi. Hingga saat ini, DNA merupakan contoh sampel yang tepat untuk teknik tersebut serta telah banyak diimaging menggunakan AFM. <\/p>\n\n\n\n

Pada gambar DNA, struktur heliks ganda dapat terlihat dengan jelas. Dalam beberapa penelitian sebelumnya, DNA diadsorpsi pada permukaan mika kemudian dimodifikasi untuk menciptakan interface positif. Hadirnya kontrol gaya interaksi antara probe dan DNA\u00a0 mengakibatkan proses imaging DNA dalam kondisi fisiologis yang lebih relevan.<\/p>\n\n\n\n

\"PeakForce
Gambar 2. PeakForce Tapping image of groove depth variations in the DNA plasmid topography obtained using the FastScan Bio AFM and FastScan-D probes (small cantilever and standard silicon tip). (A) Low-magnification AFM topography image of a plasmid showing corrugation. The white rectangle indicates the area imaged in B. (B) Higher-magnification trace (white arrow to right) and retrace (white arrow to left) images of this area showing corrugation consistent with the B form of DNA, for consecutive images. (C) Trace (solid) and retrace (dashed) height profiles taken along straight lines as indicated in B, closely following the backbone of the four plasmid scans and averaged over a 5 -pixel (~0.5) width. The height profiles confirm the observed corrugation to be the alternating major and minor grooves of double helix structure and that these grooves vary in depth along the DNA strand. The height profiles have been offset by multiples of 0.6 nm for clarity. Color scales: 3.5 nm (A), 1.1 nm (B). Reproduced with permission from Pyne et al.3<\/figcaption><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Beberapa keuntungan yang dimiliki imaging DNA melalui metode PeakForce Tapping Mode, yakni melindungi probe dan sampel dari kerusakan potensial dengan menggunakan gaya imaging rendah. Tidak hanya itu, metode ini dapat memudahkan proses imaging fluida lingkungan.<\/p>\n\n\n\n

Tentunya, hal ini berbeda dengan metode Tapping Mode yang mana tidak ada kebutuhan untuk mengatur kantilever dan pengoptimalan otomatis dalam metode ScanAsyst meningkatkan kecepatan dan konsistensi imaging.<\/p>\n","protected":false},"excerpt":{"rendered":"

Molekul biologis pertama yang dividualisasikan oleh Atomic Force Microscopy (AFM) ialah DNA. Hal ini dilakukan untuk mempelajari struktur, topologi, dinamika DNA, dan bagaimana interaksinya dengan protein. Teknologi PeakForce Tapping Mode ekslusif dari Bruker dapat melakukan imaging double helix DNA dengan resolusi tinggi yang dapat mengukur kekuatan imaging tanpa memerlukan probe khusus ataupun design AFM yang […]<\/p>\n","protected":false},"author":8,"featured_media":8274,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[236],"tags":[280,282,281],"yoast_head":"\nTeknologi PeakForce Tapping Mode untuk Imaging DNA -<\/title>\n<meta name=\"description\" content=\"Teknologi PeakForce Tapping Mode dari Bruker dapat melakukan imaging double helix DNA tanpa memerlukan probe. 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